ispltaИзвестия Санкт-Петербургской лесотехнической академииIzvestia Sankt-Peterburgskoj lesotehniceskoj akademii2079-43042658-5871СПбГЛТУ10.21266/2079-4304.2024.251.123-140isplta-421Research ArticleЛЕСНОЕ ХОЗЯЙСТВОFORESTRYК изучению роли вершинного короеда Ips acuminatus в трансмиссии фитопатогенных нематод рода BursaphelenchusThe research of the role of the sharp-toothed bark beetle Ips acuminatus in the transmission of phytopathogenic nematodes of the genus Bursaphelenchushttps://orcid.org/0000-0002-7937-4667ЧалкинА. А.ChalkinA. A.

ЧАЛКИН Андрей Андреевич – научный сотрудник отдела лесного карантина

140150, ул. Пограничная, д. 32, р. п. Быково, г. о. Раменский, Московская обл.

Web of Science Researcher ID: AET-9683-2022

Scopus AuthorID: 57220116459

CHALKIN Andrey A. – Researcher at the Forest Quarantine Department

Web of Science Researcher ID: AET-9683-2022;

Scopus Author ID: 57220116459

140150. Pogranichnaya str. 32. Bykovo. Ramensky district. Moscow region

chalkin10@ya.ruhttps://orcid.org/0000-0002-7531-4982КулиничО. А.KulinichO. A.

КУЛИНИЧ Олег Андреевич – главный научный сотрудник, начальник отдела лесного карантина, доктор биологических наук

140150, ул. Пограничная, д. 32, р. п. Быково, г. о. Раменский, Московская обл.

Web of Science Researcher ID: А-5534-2016

Scopus Author ID: 11940984400

KULINICH Oleg A. – DSc (Biological), Chief Scientific Associate, Head of Forest Quarantine Department

Web of Science Researcher ID: А-5534-2016;

Scopus Author ID: 11940984400

140150. Pogranichnaya str. 32. Bykovo. Ramensky district. Moscow region

okulinich@mail.ruhttps://orcid.org/0000-0002-1659-0258КозыреваН. И.KozyrevaN. I.

КОЗЫРЕВА Наталья Ивановна – научный сотрудник отдела лесного карантина, кандидат биологических наук

140150, ул. Пограничная, д. 32, р. п. Быково, г. о. Раменский, Московская обл.

Web of Science Researcher ID: JWP-5077-2024

Scopus Author ID: 57190217222

KOZYREVA Natalya I. – PhD (Biological), Researcher at the Forest Quarantine Department

Web of Science Researcher ID: JWP-5077-2024;

Scopus Author ID: 57190217222

140150. Pogranichnaya str. 32. Bykovo. Ramensky district. Moscow region

nkozyreva014@gmail.comhttps://orcid.org/0000-0002-0547-2547АрбузоваЕ. Н.ArbuzovaE. N.

АРБУЗОВА Елена Николаевна – старший научный сотрудник отдела лесного карантина, кандидат биологических наук

140150, ул. Пограничная, д. 32, р. п. Быково, г. о. Раменский, Московская обл.

Web of Science Researcher ID: I-7153- 2015

Scopus Author ID: 57222500674

ARBUZOVA Elena N. – PhD (Biological), Senior Researcher at the Forest Quarantine Department

Web of Science Researcher ID: I-7153-2015;

Scopus Author ID: 57222500674

140150. Pogranichnaya str. 32. Bykovo. Ramensky district. Moscow region

e.n.arbuzova@mail.ruФГБУ «ВНИИКР»РоссияFederal State Budgetary Institution «VNIIKR»Russian Federation2024270120250251123140Copyright © Чалкин А.А., Кулинич О.А., Козырева Н.И., Арбузова Е.Н., 20252025Чалкин А.А., Кулинич О.А., Козырева Н.И., Арбузова Е.Н.Chalkin A.A., Kulinich O.A., Kozyreva N.I., Arbuzova E.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://izvestiya-lta.spbftu.ru/jour/article/view/421

Одна из потенциальных угроз для хвойных лесов Российской Федерации – распространение вилта хвойных пород, вызываемого сосновой стволовой нематодой Bursaphelenchus xylophilus. Данный карантинный организм пока не выявлен в России, но распространен в соседних странах: Китае, Японии, Южной Кореи. В естественных сосновых насаждениях распространение B. xylophilus осуществляется усачами рода Monochamus, однако увядающие деревья также заселяются другими видами насекомых-ксилофагов, в частности, короедами рода Ips. Представлены результаты исследований, проведенных в контролируемых лабораторных условиях, по возможности переноса вершинным короедом Ips acuminatus нематод B. xylophilus. С этой целью два типа бревен сосны (Pinus sylvestris), заселенные короедом I. acumunatus (I тип) и искусственно зараженные B. xylophilus (II тип), были помещены вместе в один садок. В результате установлено, что жуки заселили зараженные нематодой бревна сосны, а через 25 суток 47% отродившихся в зараженной древесине молодых жуков содержали личинок нематод B. xylophilus. Кроме этого вида, в жуках и древесине были выявлены нематоды B. paraburgeri, B. doui, а также Teratorhabditis synpapillata, Micoletzkya synpapillata. Идентификация нематод проводилась с применением молекулярно-генетических методов диагностики. Наибольшее количество нематод в жуках (91%) находилось в среднегрудном отделе короедов под надкрыльями. При проведении смыва водой с поверхности жуков нематод не зафиксировано. Результаты исследований дают основание предполагать, что вершинный короед I. аcuminatus может быть переносчиком патогенных нематод B. xylophilus.

One of the potential threats to coniferous forests of the Russian Federation is the spread of pine wilt disease caused by the pinewood nematode Bursaphelenchus xylophilus. This quarantine organism has not yet been detected in Russia, but is widespread in neighbouring countries: China, Japan, South Korea. In natural pine plantations the spreading of B. xylophilus is carried out by Monochamus beetles, but stressed trees are also infeсted by other insects, in particular by bark beetles of the genus Ips. The results of studies conducted under controlled laboratory conditions on the possibility of transfer of B. xylophilus nematodes by the bark beetle Ips acuminatus are presented. For this purpose, two types of pine (Pinus sylvestris) logs with I. acumunatus (type I) and artificially infested with B. xylophilus (type II) were placed together in one cage. I. acuminatus beetles were found in nematode-infested pine logs, and after 25 days, 47% of the emerging beetles in the infested wood contained the B. xylophilus juveniles. The nematodes B. paraburgeri, B. doui, Teratorhabditis synpapillata, Micoletzkya synpapillata were also found in I. acuminatus beetles and wood. The nematodes were identified using molecular genetic diagnostic methods. The highest number of nematodes in beetles (91%) was found in the mid-thoracic section of bark beetles under the elytra. No nematodes were found in the beetles during water flushing from the beetle surface. The results suggest that the bark beetle I. acuminatus may be a vector by pathogenic nematodes B. xylophilus.

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The authors declare that there are no conflicts of interest present.