The effect of humic preparations on seed germination, the number of cotyledons and the growth of seedlings of Pinus sylvestris

Growth stimulators accelerate seed germination, increase germination, have a positive effect on the development of the aboveground part and the root system of seedlings, increase survival during transplantation and contribute to better survival in extreme conditions. Currently, there are a large number of different substances with a growth-stimulating effect, but preference is given to environmentally friendly. Such stimulants include humic preparations. The aim of the study was to study the effect of humic preparations on laboratory germination of seeds, on the change in the number of cotyledons in shoots and the growth of seedlings of Pinus sylvestris. Pine seeds were soaked in solutions of preparations: Ecorost, Gumi, Humate + 7 iodine, Humate + 9 trace elements and in water (control). The germination of treated seeds was studied under laboratory conditions. We also took into account the number of cotyledons at each shoot in experimental versions and on control. In the greenhouse conditions of the Arkhangelsk region, the seedlings of Pinus sylvestris were watered with solutions of humic preparations Ecorost, Gumi, Humate +7. In the experiment on laboratory germination of Pinus sylvestris seeds, there were no significant differences between the control and experimental variants. According to the results of the evaluation of the structure of seedlings by the number of cotyledons, depending on the treatment of seeds with various humic preparations (Ecorost, Gumi, Humate +7, Humate + 9 trace elements), it was found that these preparations do not have an effect on the number of cotyledons in shoots of Pinus sylvestris. Seedlings of Pinus sylvestris of the first year of cultivation, fertilizing with humic preparation Humate + 7, were 0.3 cm higher than the control variant, which is not a significant indicator for the use of this substance in production. Preparations of Gumi and Ecorost showed a depressing effect on the growth of seedlings. All the tested preparations had no effect on the radial increase. Thus, the humic preparations tested in this study with the concentration selected by us do not affect the increase in seed germination, the growth of seedlings of Pinus sylvestris, or have a slight excess. Therefore, research in the field of application of specific humic preparations, their concentrations, soaking time, watering frequency, etc. to increase the efficiency of growing the planting material of Pinus sylvestris, should be continued.

1. Adilbayeva Zh., Maisupova B., Abayeva K., Utebekova A., Akhmetov R. The effect of stimulants on the seed germination and growth of Schrenk's spruce seedlings. OnLine Journal of Biological Sciences, 2021, vol. 21, pp. 354–364. DOI: 10.3844/ojbsci.2021.354.364.

2. Bajtulin I.O. Sozdanie lesnogo pitomnika i tehnologija vyrashhivanija posadochnogo materiala [Creation of a forest nursery and the technology of growing planting material]. Kostanay: Kostanajpoligrafija, 2009. 48 p. (In Russ.)

3. Galdina T.E., Har'kova V.A. Ocenka vlijanija biostimuljatorov na sostojanie i kachestvo sejancev sosny obyknovennoj (Pinus silvestris L.) [Evaluation of the effect of biostimulants on the condition and quality of seedlings of scots pine (Pinus sylvestris L.)]. IV International Student Electronic Scientific Conference «Student Scientific Forum». Moscow, 15 February – 31 March, 2012. URL: https://scienceforum.ru/2012/article/2012000558 (September 14, 2022). (In Russ.)

4. Gavrilova O.I., Gostev K.V., Gostev V.A., Zhuravleva M.V., Rumjanceva M.A. Issledovanie vlijanija predposevnoj obrabotki semjan drevesnyh porod vodoj, aktivirovannoj plazmoj [Investigation of the effect of pre-sowing treatment of wood y seeds with water activated by plasma]. Inzhenernyj Vestnik Dona, 2016, vol. 4. URL: https://www.elibrary.ru/download/elibrary_28945302_98815896.pdf (accused September 14, 2022). (In Russ.)

5. Gorbunova S.V., Sen`kov A.O., Fajzulin D.X. Opy`t primeneniya guminovogo preparata pri vy`rashhivanii seyancev xvojny`x porod s zakry`toj kornevoj sistemoj v usloviyax Arxangel`skoj oblasti [The experience of using a humic preparation in the cultivation of coniferous seedlings with a closed root system in the conditions of the Arkhangelsk region]. Sibirskij lesnoj zhurnal, 2022, vol. 1. pp. 41–51. (In Russ.)

6. Grehova I.V., Matveeva N.V. Reakcija jarovoj pshenicy na primenenie reguljatorov i mikroudobrenija pri protravlivanii semjan [Reaction of spring wheat to the use of regulators and micro-fertilizers during seed pickling]. Agrarnyj vestnik Urala, 2014, vol. 1, pp. 6–8. (In Russ.)

7. Himanen K., Helenius P., Nygren M. Liotuska sittelyiden vaikutus kuusen siementen ita¨miseen [Effect of pre-sowing soaking treatments on germination of Norway spruce seeds]. Metsa tieteen aikakauskirja, 2010, vol. 2, pp. 103–114.

8. Himanen K., Lilja A., Poimala née Rytkönen A., Nygren M. Soaking effects on seed germination and fungal infection in Picea abies. Scandinavian Journal of Forest Research, 2013, vol. 28, pp. 1–7.

9. Kabanova C.A., Danchenko M.A., Borcov V.A., Kocherganov I.S. Rezul'taty predposevnoj obrabotki semjan sosny obyknovennoj stimuljatorami rosta [Results of pre-sowing treatment of Scots pine seeds with growth stimulants]. Lesotehnicheskij zhurnal, 2017, vol. 2, pp. 75–83. (In Russ.)

10. Kabanova S.A., Danchenko M.A., Shishkin A.M., Krizhanovskaja E.I., Primenenie rostovyh veshhestv dlja vyrashhivanija posadochnogo materiala sosny obyknovennoj [The use of growth substances for the cultivation of Scots pine planting stock]. Vestnik Povolzhskogo gosudarstvennogo tehnologicheskogo universiteta, 2019, vol. 2, pp. 52–61. (In Russ.)

11. Kazakov V.I., Prokazin N.E., Lobanova E.N., Kazakov I.V. Vliyanie sortirovki semyan xvojny`x porod na posevny`e kachestva [The effect of sorting coniferous seeds on sowing qualities]. Lesotexnicheskij zhurnal, 2016, vol. 3, pp. 161–167. (In Russ.)

12. Mitrofanov S.V., Gapeeva N.N., Mochalova E.N. Vlijanie guminovyh udobrenij na posevnye kachestva Eli evropejskoj [Influence of humic fertilizers on the sowing quality of European spruce]. Jekologicheski ustojchivoe zemledelie: sostojanie, problemy i puti ih reshenija [Environmentally Sustainable Agriculture: State, Problems and Ways to Solve Them]: Proceedings of the All-Russian Scientific-practical Conference with International Participation. VNIIOU – filial FGBNU «Verhnevolzhskij FANC», Ivanovo, 22–24 June 2018). Ivanovo: PresSto, 2018, pp. 177–181. (In Russ.)

13. Nemkov P.S., Grehova I.V. Vlijanie guminovogo preparata na sejancy hvojnyh porod [Influence of a humic preparation on coniferous seedlings]. Teoreticheskaja i prikladnaja jekologija, 2015, vol. 1, pp. 96–99. (In Russ.)

14. Novosel'ceva A.I., Smirnov N.A. Spravochnik po lesnym pitomnikam (Handbook of forest nurseries), Moscow: Lesnaja prom-st', 1983, 280 p. (In Russ.)

15. Ostroshenko V.V., Ostroshenko L.Ju., Kljuchnikov D.A., Ostroshenko V.Ju., Chekushkina T.N. Vlijanie stimuljatorov rosta na jenergiju prorastanija i laboratornuju vshozhest' semjan sosny obyknovennoj (Pinus silvestris L.) [The effect of growth stimulants on the germination energy and laboratory germination of the seeds of Scots pine (Pinus silvestris L.)]. Izvestija Samarskogo nauchnogo centra Rossijskoj akademii nauk, 2015, vol. 17, no. 6, pp. 242–247. (In Russ.)

16. Pirogovskaja G.V., Bogomaz I.A., Shagieva E.I., Kovalenok M.F., Kuleshova S.I., Naumova G.V., Rajcina G.I. Ispol'zovanie guminovyh preparatov v poluchenii mineral'nyh udobrenij [The use of humic preparations in the production of mineral fertilizers]. Guminovye veshhestva v biosfere, Moscow: Nauka, 1993, pp. 166–173. (In Russ.)

17. Popov V.Ja., Fajzulin D.H. Otbor jelitnyh derev'ev sosny obyknovennoj [Selection of elite Scots pine trees]. Arkhangelsk: NRIF, 2001. 23 p. (In Russ.)

18. Popov V.Ja., Tuchin P.V., Fajzulin D.H. Otbor populjacij eli obyknovennoj s cennymi nasledstvennymi svojstvami [Selection of populations of common spruce with valuable hereditary properties]. Lesovodstvenno-jekonomicheskie voprosy vosproizvodstva lesnyh resursov Evropejskogo Severa [Forestry and economic issues of reproduction of forest resources of the European North], Arkhangelsk, 2000, pp. 83–88. (In Russ.)

19. Popov V.Ja., Zharikov V.M. Izmenchivost' predstavlennosti osobej s razlichnym chislom semjadolej v potomstve otdel'nyh derev'ev i populjacij sosny obyknovennoj [Variability of the representation of individuals with different numbers of cotyledons in the offspring of individual trees and populations of Scots pine]. Tezisy dokladov na otchetnoj sessii laboratorii lesovodstva i lesovedenija za 1970 god [Abstracts of Reports at the Reporting Session of the Laboratory of Forestry and Forestry Science for 1970], Arkhangelsk, 1971, pp. 18–21. (In Russ.)

20. Popov V.Ja., Zharikov V.M. Metody otbora i rannej diagnostiki nasledstvennyh svojstv pljusovyh derev'ev sosny i eli [Methods of selection and early diagnosis of hereditary properties of plus trees of pine and spruce]. Arkhangelsk, 1973. 39 p. (In Russ.)

21. Popov V.Ja., Zharikov V.M. Rannjaja diagnostika nasledstvennyh svojstv pljusovyh derev'ev sosny i eli: metodicheskoe posobie [Early diagnosis of hereditary properties of pine and spruce plus trees: a methodological guide: method book]. Arkhangelsk: AILiLH, 1978, 14 p. (In Russ.)

22. Prokazin N.E., Lobanova E.N., Pentel'kina N.V., Ivanjusheva G.I., Sahnov V.V., Petrov V.A., Chukarina A.V., Bagaev S.S. Vyrashhivanie posadochnogo materiala hvojnyh porod s ispol'zovaniem rostovyh stimuljatorov [Cultivation of coniferous planting stock using growth stimulants]. Lesohozjajstvennaja informacija, 2015, vol. 1, pp. 50–56. (In Russ.)

23. Smirnov A.I., Orlov F.S., Drozdov I.I. Priemy intensivnoj agrotehniki pri poseve semjan hvojnyh vidov [Intensive agricultural techniques for sowing seeds of coniferous species]. Lesnoj vestnik, 2015, vol. 2, pp. 69–73. (In Russ.)

24. Suvorov V.I. Jeffektivnost' predposevnoj podgotovki semjan hvojnyh porod pri sozdanii kul'tur metodom poseva na vyrubkah Urala [The effectiveness of pre-sowing preparation of coniferous seeds in the creation of crops by sowing in the cuttings of the Urals]. Lesa Urala i hozjajstvo v nih, 1968, vol. 1, pp. 371–392. (In Russ.)

25. Sviridov L.T., Gomzyakov N.D., Novikov A.I., Tomilin A.N. O novom perspektivnom texnologicheskom komplekse mashin i oborudovaniya dlya obrabotki lesny`x semyan [About a new promising technological complex of machines and equipment for processing forest seeds]. Lesnoj zhurnal, 2009, vol. 5, pp. 31–37. (In Russ.)

26. Teply`x A.A., Proxorova E.V. Vliyanie srokov xraneniya semyan eli na ix texnicheskuyu vsxozhest`. Vestnik Povolzhskogo gosudarstvennogo texnologicheskogo univepsiteta. Ser.: Les. E`kologiya. Prirodopol`zovanie, 2018. vol. 2 (38). pp. 19–28. DOI: 10.15350/2306-2827.2018.2.19. (In Russ.)

27. Ustinova T.S. Vlijanie preparata Gumat +7 na rost sejancev eli evropejskoj [The effect of the preparation Gumat + 7 on the growth of European spruce seedlings]. Aktual'nye problemy lesnogo kompleksa, 2009, vol. 22, pp. 146–148. (In Russ.)

28. Ustinova T.S., Zurov R.N. Vlijanie preparata Gumat+7 na rostovye processy hvojnyh porod [The effect of the preparation Gumat + 7 on the growth processes of conifers]. Aktual'nye problemy lesnogo kompleksa, 2010, vol. 26, pp. 115–118. (In Russ.)

29. Yakimenko O.S., Terekhova V.A. Guminovye preparaty i ocenka ih biologicheskoj aktivnosti dlya celej sertifikacii [Humic preparations and assessment of their biological activity for certification purposes]. Pochvovedenie, 2011, vol. 11, pp. 1334–1343. (In Russ.)

30. Zhigunov A.V. Teorija i praktika vyrashhivanija posadochnogo materiala s zakrytoj kornevoj sistemoj [Theory and practice of growing planting material with a closed root system]. St. Petersburg: SPbNIILH, 2000. 294 p. (In Russ.)