Complex neutral sulfite liquor lignosulfonate processing to feed bioproducts

In the cellulose production from birch wood by the neutral sulfite method liquors are formed. These liquors contain organic and mineral substances. At present, minerals content of the neutral sulfite liquors is not regenerated. Organic compounds especially carbohydrate oligomers are also not used anywhere. The only way currently available at the enterprises to utilize these liquors is to obtain lignosulfonates, which indicates the economic irrationality of the resource usage. The paper considered the possibility of separating lignin and lignosulfonate carbohydrates from neutral sulfite liquors by a biocatalytic method with the formation of simple sugars. Subsequently these simple sugars can be used for microbiological synthesis of lactic acid by the filamentous fungus Rhizopus oryzae F-1030. There are also wastes formed after the lactic acid separation, which also need to be disposed of. These wastes consist of the culture liquid based on the lignosulfonate enzyme lysate and the biomass of the fungus. As a possible way of their utilization, it is proposed to use the insoluble fraction of lignosulfonate lignin and the fungus R. oryzae F-1030 biomass, rich with chitin-glucan, to obtain feed additives for farm animals. These additives must have adsorption properties to mycotoxins. It was shown that the treatment of lignosulfonate with enzyme preparations Accellerase XC and Accellerase XY leads to an increase in the content of simple sugars in hydrolysates, which can be used by microorganisms as carbon sources. It was found that the fungus R. oryzae F-1030 synthesizes lactic acid at an average concentration of 42.0±4.0% per 1 g of assimilated RS during its cultivation on neutral sulfite lignosulfonate enzyme lysates. During this process, 1.8±0.2 g/dm³ of fungus dry biomass is also formed. It was proved the possibility of using neutral sulfite lignosulfonate lignin and the biomass of the fungus R. oryzae F-1030 in a 1:1 ratio to obtain a feed additive with an adsorption capacity for mycotoxins.

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