Analysis of the polymorphism of microsatellite loci in Picea abies (L.) H. Karst. and Picea obovatа Ledeb populations
https://doi.org/10.21266/2079-4304.2021.237.97-108
Abstract
The processes of genesis of coniferous forests are widely investigating using molecular markers. Many studies are based on the analysis of microsatellite loci, which are acknowledged as the most reproducible and polymorphic co-dominant molecular markers. This study is aimed to establish the possibility of using microsatellite markers to determine differentiation between Norway and Siberian spruce (Picea abies and Picea obovata). Another task of this research was to assess the degree of genetic isolation of regional spruce population in the territory of European part of the Russian Federation. This study shows that using a large number of microsatellite markers allows to divide the sample of spruce collected from European part of Russia and Siberia in two genetic clusters, corresponding to Picea abies and Picea obovata. The microsatellite loci analyzed in the study can be used to assess genetic diversity and geographic origin of spruce trees and determine the origin of wood and planting material of Norway and Siberian spruce.
About the Authors
V. A. Volkov
St.Petersburg State Forest Technical University
Russian Federation
Russian Federation
VOLKOV Vladimir A. – PhD student
194021. Institute per. 5. St. Petersburg
G. V. Kalko
Saint-Petersburg forestry research institute
Russian Federation
Russian Federation
KALKO Galina V. – head of the research laboratory
194021. Institutsky per. 21. St. Petersburg
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Review
For citations:
Volkov V.A., Kalko G.V. Analysis of the polymorphism of microsatellite loci in Picea abies (L.) H. Karst. and Picea obovatа Ledeb populations. Izvestia Sankt-Peterburgskoj lesotehniceskoj akademii. 2021;(237):97-108. (In Russ.) https://doi.org/10.21266/2079-4304.2021.237.97-108
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