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Application of KASP and HRM markers for detecting polymorphisms affecting adaptive traits in Scots pine and Norway spruce

https://doi.org/10.21266/2079-4304.2024.251.229-244

Abstract

Climate change significantly impacts forest ecosystems, particularly coniferous species such as Scots pine (Pinus sylvestris) and Norway spruce (Picea abies), which form the basis of temperate forests. The adaptation of these species to new environmental conditions necessitates consideration of the genetic variability of key adaptive traits. This study investigates single nucleotide polymorphisms (SNPs) in candidate genes that regulate photoperiodic response and circadian rhythms, which are critical to tree physiological processes. The research describes the development and testing of molecular markers based on KASP (Kompetitive Allele Specific PCR) and HRM (High Resolution Melting) methods for high-throughput genotyping. SNPs were identified in the photoreceptor genes PhyO and CRY1 and the photoperiodic response genes PRR1 and PRR7. These polymorphisms exhibit geographic clinality, highlighting their significant role in tree adaptation to latitudinal gradients of light and temperature conditions. The findings confirm the efficiency of KASP and HRM methods for genotyping, enabling the use of these markers for monitoring genetic diversity and selecting resilient genotypes in breeding programs. Such approaches ensure the integration of genetically adapted planting material into reforestation efforts, which is particularly relevant in the context of changing climate conditions.

About the Authors

V. A. Volkov
St.Petersburg State Forest Technical University; Saint-Petersburg Forestry Research Institute
Russian Federation

VOLKOV Vladimir. A. – PhD (Biological), Director of the Center for Bioinformatics and Genomic Research; researcher of the Research Department of Genetics and Biotechnology

194021. Institute per. 5. Let. U. St. Petersburg

194021. Institutskiy av. 21. St. Petersburg



D. S. Karzhaev
St.Petersburg State Forest Technical University; Saint-Petersburg Forestry Research Institute
Russian Federation

KARZHAEV Dmitry S. – junior researcher at the Centre for Bioinformatics and Genomic Research, PhD student of SPbFTU; junior researcher of the Research Department of Genetics and Biotechnology

194021. Institute per. 5. Let. U. St. Petersburg

194021. Institutskiy av. 21. St. Petersburg



E. D. Safronycheva
Saint-Petersburg Forestry Research Institute
Russian Federation

SAFRONYCHEVA Elizaveta D. – Junior researcher of the Research Department of Genetics and Biotechnology

194021. Institutskiy av. 21. St. Petersburg



M. V. Tis
Saint-Petersburg Forestry Research Institute
Russian Federation

TIS Margarita V. – Research assistant of the Research Department of Genetics and Biotechnology

194021. Institutskiy av. 21. St. Petersburg



K. V. Chukhlantseva
Saint-Petersburg Forestry Research Institute
Russian Federation

CHUKHLANTSEVA Ksenia V. – Research assistant of the Research Department of Genetics and Biotechnology

194021. Institutskiy av. 21. St. Petersburg



V. V. Sharshavikova
Saint-Petersburg Forestry Research Institute
Russian Federation

SHARSHAVIKOVA Veronika V. – Research assistant of the Research Department of Genetics and Biotechnology

194021. Institutskiy av. 21. St. Petersburg



E. K. Potokina
Saint-Petersburg Forestry Research Institute
Russian Federation

POTOKINA Elena K. – DSc (Biological), Senior researcher of the Research Department of Genetics and Biotechnology

194021. Institutskiy av. 21. St. Petersburg



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Review

For citations:


Volkov V.A., Karzhaev D.S., Safronycheva E.D., Tis M.V., Chukhlantseva K.V., Sharshavikova V.V., Potokina E.K. Application of KASP and HRM markers for detecting polymorphisms affecting adaptive traits in Scots pine and Norway spruce. Izvestia Sankt-Peterburgskoj lesotehniceskoj akademii. 2024;(251):229-244. (In Russ.) https://doi.org/10.21266/2079-4304.2024.251.229-244

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ISSN 2079-4304 (Print)
ISSN 2658-5871 (Online)