Experience in clonal micropropagation of the selected form of Vaccinium angustifolium Aiton

The article presents the results of research on the clonal micropropagation of the form of narrow-leaved blueberry (Vaccinium angustifolium Aiton) collected at the variety testing site of a plantation in the Arkhangelsk region. For clonal micropropagation of species, forms and varieties, it is necessary to select an individual protocol corresponding to the biological characteristics of the replicated species. For the initial treatment of plant material in vivo, the first 10 cm of annual growth of the donor plant were cut off. At the sterilization stage, when introducing into the in vitro culture, a 5% solution of “Lysoformin 3000” and a 5% solution of “Brilliant” were used to sterilize the explants of narrow-leaved blueberry at an exposure time of 5, 10 and 15 minutes. The largest proportion of healthy explants was recorded when using “Lysoformin 3000” solution for 15 minutes – 76.6%. At the stage of microreproduction proper, when hormones 2-ip (1.0-1.5 ml/l) and indolylacetic acid (0.1-0.2 ml/l) were added to the nutrient medium, the largest average number of formed buds and the highest values of the average length of micro-shoots of narrow-leaved blueberries were marked. The period between passages lasts 35 days. When replicating regenerants with segments containing 1 bud each, the reproduction coefficient is 3. In order to increase the amount of planting material yield, the upper part of the plant was cut off for further replication, and the rooted segment with two buds was moved to the vegetation plant for adaptation. The adaptation of micro-plants was carried out according to a proven technique in a vegetation installation for 16 days, which allows you to save the maximum number of adaptable plants.

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