The use of environmentally friendly preparations to stimulate the growth and development of Pinus Sylvestris L. seedlings

The study of the effect of growth stimulants on the quality of planting material for reforestation purposes has great practical importance. Growth-stimulating drugs help accelerate the processes associated with the growth and development of plants, as well as improve their adaptation to environmental factors. The research was carried out in the protected soil of the Vologda Breeding and Seed Forestry Center nursery, located in Kadnikov, Vologda region. As stimulators of seed germination and activation of seedlings of Pinus sylvestris L. technical lignosulfonates (chlorinated and nitrosated) of 10% concentration; birch bark extract (fraction less than 1 mm and fraction more than 1 mm) with a concentration of 1:10000; filtrate obtained by separating suberin from birch bark with a concentration of 1:10000; “Flavobacterin”; “Mizorin”; condensate formed during drying of lumber; the ashes of Ejyafyadlajyokyudl volcano were used. It was found that by 10-15th day of cultivation, the best results were shown by seedlings of seeds treated with chlorinated and nitrosated lignosulfonate solutions (70-94% of seedlings). Seedlings of seeds treated with filtrate solutions from the release of suberin from birch bark, condensate from drying wood, ash from the Ejyafyadlajyokyudl volcano, “Mizorin” and “Flavobacterin” prevail 1.5 times on the 15th day after sowing compared with the control variant. It was noted that the highest average height (12.30±0.32 cm) was observed in seedlings treated with a solution of chlorinated lignosulfonates; the maximum average diameter (2.19±0.25 mm) and root length (17.90±0.26 cm) were recorded in pine specimens treated with “Flavobacterin”. It was found that the yield of standard seedlings increased to 96–99% with the use of lignosulfonates, extracts of birch bark fraction less than 1 mm, condensate solutions, “Mizorin”, “Flavobacterin” and ash from the Ejyafyadlajyokyudl volcano, while in the control area, this figure was only 40%. If the recommended methods of using stimulants are followed, the need for seedling growth is eliminated, which significantly reduces the cost of planting material production.

1. Agro-climatic resources of the Vologda region. Leningrad: Hydrometeoizdat, 1972. 185 p. (In Russ.)

2. Antipov N.P. Climate. Nature of the Vologda region. Vologda, 1957, pp. 111– 135. (In Russ.)

3. Avdoshenko A.K. Seasonal rhythmics of Vologda nature: manuscript. Vologda, 1980. 85 p. (In Russ.)

4. Babich N.A., Barabin A.I., Tutygin G.S. The state and problems of reforestation in the European North. Collection of scientific papers. Arkhangelsk, 1999, pp. 4–12. (In Russ.)

5. Barber J.J. Resent advances in planting techniques. Forest Farmer, 1972, vol. 32, 26 p.

6. Belyaev V.V. The state and main directions of increasing the efficiency of reforestation in the European North of Russia. Bulletin of the Pomor University, 2001, iss. 2, pp. 45-50. (In Russ.)

7. Calvo P., Nelson L., Kloepper J.W. Agricultural uses of plant biostimulants. Plant and Soil, 2014, vol. 383, iss. 1-2, pp. 3-41. DOI: 10.1007/s11104-014-2131-8.

8. Climate of Russia. St. Petersburg: Hydrometeoizdat, 2001. 654 p. (In Russ.)

9. Davis A.S., Jacobs D.F. Quantifying root system quality of nursery seedlings and relationship to outplanting performance. New Forests, 2005, vol. 30, pp. 295–311. DOI: 10.1007/s11056-005-7480-y.

10. Drochkova A.A. Analysis of the effect of lignosulfonates on germination and germination energy of Pinus sylvestris L. seeds. Papanin readings: articles by participants of the int. youth sci. conf. Arkhangelsk, 2017, pp. 52-55. (In Russ.)

11. Drochkova A.A. Analysis of the effects of condensate formed during the drying of lumber on the germination and germination energy of Pinus sylvestris L. seeds. Actual problems of the development of the forest complex: proceedings of the XVIII int. sci.- tech. conf. Vologda, 2020, pp. 31-32. (In Russ.)

12. Drochkova A.A. Analysis of the influence of the ash of the Eyjafjallajökull volcano on the main quality indicators of Pinus sylvestris L. seeds. Fundamental and applied scientific research: current issues, achievements and innovations: collection of articles of the XLIV int. sci.-pract. conf. Penza, 2021, pp. 137–139. (In Russ.)

13. Egorova A.A., Babich N.A. The effect of Flavobacterin on germination and germination energy of pine seeds. Ecological problems of the Arctic and Northern territories: an interuniversity collection of scientific papers, 2012, iss. 15, pp. 89–91. (In Russ.)

14. Gordeeva D.V. The effectiveness of growth stimulants in the cultivation of seedlings of Scots pine with a closed root system. Engineering personnel is the future of Russia's innovative economy, 2023, no. 1, pp. 188-191. DOI: 10.34220/issn.2222-7962/2019.3/8. (In Russ.)

15. Grossnickle S.C. Why seedlings survive: influence of plant attributes. New Forests, 2012, vol. 43, pp. 711–738. DOI: 10.1007/s11056-012-9336-6.

16. Grossnickle S.C., MacDonald J.E. Why seedlings grow: influence of plant attributes. New Forests, 2018, vol. 49, pp. 1–34. DOI: 10.1007/s11056-017-9606-4.

17. Hoffmann F. Untersuchungen zur Stickstoffernährung junger Koniferen. Archiv für Forstvesen, 1966, b. 10, ss. 1093-1103.

18. Igaunis G.A. Cultivation of planting material in greenhouses with synthetic coating. Moscow: Lesn. prom-st', 1974. 236 p. (In Russ.)

19. Ivanov V.P. Long-term growth stimulation as an effect of increasing the stability of Scots pine under the influence of PABA. Russian forestry journal, 2001, no. 5-6, pp. 192-195. (In Russ.)

20. Louder R. Volcanic ash may be helping vegetation growth. Reykjavik: Grapevine, 2010. 24 p.

21. Mochalov B.A. Development of forestry in the north of Russia. Science – to the forestry of the North. Arkhangelsk, 1999, pp. 56-60. (In Russ.)

22. Mochalov B.A., Sinnikov A.C. Recommendations for the cultivation of coniferous planting material in polyethylene greenhouses. Arkhangelsk, 1977. 12 p. (In Russ.)

23. Mochalov B.A., Krestsova V.P., Drannikova V.A., Mochalova G.A., Chechueva T.A. Cultivation of breeding improved planting material in greenhouses. Materials of the reporting session on the results of scientific research for 1990. Arkhangelsk, 1991, pp. 16–18. (In Russ.)

24. Ostroshenko V.Yu., Ostroshenko L.Yu. The effect of stimulants on seed germination and the growth of seedlings of Scots pine (Pinus sylvestris L.). BHEI. Russian forestry journal, 2023, no. 4(394), pp. 93–104. DOI: 10.37482/0536-1036-2023-4-93-104. (In Russ.)

25. Pentelkin S.K. The use of safe chemicals in the cultivation of pine seedlings. Forestry information, 2000, pp. 16-19. (In Russ.)

26. Pentelkin S.K. Results of the study of growth stimulators and polymers in forestry over the past 20 years. Forestry information, 2003, no. 10, pp. 34-53. (In Russ.)

27. Pentelkina N.V. Problems of growing planting material in forest nurseries and ways to solve them. Actual problems of the forest complex, 2012, no. 31, pp. 189-193. (In Russ.)

28. Pentelkin S.K., Pentelkina N.V. Studying the possibility of using new growth regulators in the cultivation of coniferous seedlings. Improving scientific support for forestry production: tez. dokl. the All-Union sci.-pract. conf. of young scientists, graduate students and specialists of the branch. Pushkino, 1990, 93 p. (In Russ.)

29. Pigarev F.T., Belyaev V.V., Sungurov R.V. Comprehensive assessment of the quality of planting material and its application in the European North: method. guidelines. Arkhangelsk, 1987. 16 p. (In Russ.)

30. Popova N.Ya., Rodin A.R., Sharamko D.V. Problems and prospects of using growth regulators in forest nurseries. Collection of sci. works of MLTI, 1991, iss. 245, pp. 12-16. (In Russ.)

31. Rikala R. Seedling production for reforestation. Forest regeneration in the Northern Parts of Europe: proceedings of the Finnish-Russian Forest Regeneration Seminar. Vuokatti, 2000, pp. 127-140.

32. Rodin A.R. Spruce crops in logging areas. Moscow: Lesn. pro-'st, 1977. 169 p. (In Russ.)

33. Rodin A.R., Popova N.Ya. Prospects of using environmentally friendly preparations in forest nurseries. Russian forestry journal, 1991, no. 6, pp. 12-16. (In Russ.)

34. Rodin A.R., Popova N.Ya., Kandyba E.V. Highly effective preparations for nurseries. Forestry, 1997, no. 1, pp. 28-30. (In Russ.)

35. Runova E.M., Denisenko A.V. The effect of growth-generating drugs on the germination of seeds of the common pine (Pinus sylvestris L.). Actual problems of the forest complex, 2024, no. 65, pp. 125-128. (In Russ.)

36. Salaš P., Sasková H., Mokričková J., Litschmann T. Evaluation of Different Types of Rooting Stimulators. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2013, vol. 60, no. 8, pp. 217-228. DOI: 10.11118/actaun201260080217.

37. Shchukin R.A., Bogdanov O.E., Zavoloka I.P., Ryazanov G.S., Kruglov N.M. Biotechnological Basis for Application of Growth Regulators for Rooting of Green Cuttings of Trees and Shrubs in a Greenhouse with a Misting System. BIO Web of Conferences, 2020, vol. 23, art, no. 01009. DOI: 10.1051/bioconf/20202301009.

38. Sinnikov A.S., Mochalov B.A., Drachkov V.N. Cultivation of coniferous seedlings in polyethylene greenhouses. Moscow: Agropromizdat, 1986. 125 p. (In Russ.)

39. Sungurova N.R., Drochkova A.A. Biometric characteristics of planting material as a test indicator of the success of Pinus sylvestris L. crops. BHEI. Russian forestry journal, 2021, no. 4, pp. 107–116. DOI: 10.37482/0536-1036-2021-4-107-116. (In Russ.)

40. Sungurova N.R., Drochkova A.A., Gayevsky N.P., Volykhina N.V., Babich N.A. Wood drying condensate as an activator of germination and germination energy of Pinus sylvestris L. pine seeds. Lesnoy vestnik, 2022, vol. 26, no. 4, pp. 39–45. DOI: 10.18698/2542-1468-2022-4-39-45. (In Russ.)

41. Tyukavina O.N., Demina N.A. Biologically active preparations for stimulating the growth of coniferous seedlings. Bulletin of the Buryat State Agricultural Academy named after V.R. Filippov, 2023, no. 1(70), pp. 122–133. DOI: 10.34655/bgsha.2023.70.1.015. (In Russ.)