Genetic diversity of Siberian stone pine (Pinus sibirica Du Tour) in provenance trial plantation
https://doi.org/10.21266/2079-4304.2026.258.51-68
Abstract
The results of the assessment of the genetic diversity of Siberian stone pine (Pinus sibirica Du Tour) in provenance trial plantation on the territory of the Karaulny district Forestry of the Educational and Experimental Forestry of the Reshetnev Siberian State University of Science and Technology, are presented. The studied Siberian stone pine of different geographical origin (Yartsevskaya, Leninogorsk, Altai and Biryusinsk samples) show a moderate level of genetic diversity. Most of the genetic diversity is accumulated within samples, and interpopulation variability accounts for 2.9%. The analysis of the genetic relationships showed that the studied samples are grouped into two clusters. The first cluster was formed by the Yartsevo and Biryusinsk samples, the second by the Altai and Leninogorsk samples. The results obtained can be used in longterm monitoring of the state of genetic resources of Siberian stone pine in Siberia.
About the Authors
M. A. Sheller
Siberian State University named after M.F. Reshetnev
Russian Federation
Russian Federation
SHELLER Marina A. – PhD (Biological), Researcher, Scientific Laboratory of the Scientific Research Department
660037. Krasnoyarskiy Rabochiy av. 31. Krasnoyarsk
A. A. Ibe
Siberian State University named after M.F. Reshetnev
Russian Federation
Russian Federation
IBE Aleksey A. – PhD (Agricultural), Researcher, Scientific Laboratory of the Scientific Research Department
660037. Krasnoyarskiy Rabochiy av. 31. Krasnoyarsk
T. V. Sukhikh
Siberian State University named after M.F. Reshetnev
Russian Federation
Russian Federation
SUKHIKH Tatiana V. – Researcher, Scientific Laboratory of the Scientific Research Department
660037. Krasnoyarskiy Rabochiy av. 31. Krasnoyarsk
A. M. Pastukhova
Siberian State University named after M.F. Reshetnev
Russian Federation
Russian Federation
PASTUKHOVA Albina M. – PhD (Agricultural), Associate Professor, Department of Selection and Landscaping, Senior Researcher, Scientific Laboratory of the Scientific Research Department
660037. Krasnoyarskiy Rabochiy av. 31. Krasnoyarsk
Yu. E. Shcherba
Siberian State University named after M.F. Reshetnev
Russian Federation
Russian Federation
SHCHERBA Yulia E. – PhD (Agricultural), Associate Professor, Department of Selection and Landscaping, Senior Researcher, Scientific Laboratory of the Scientific Research Department
660037. Krasnoyarskiy Rabochiy av. 31. Krasnoyarsk
References
1. Bekh I.A., Kravets S.A., Bisirova E.M. Cedar is the Pearl of Siberia: a pop. sci. publ. Tomsk: Printing Manufactory, 2009. 50 p. (In Russ.)
2. Belokon M.M., Politov D.V., Mudrik E.A., Polyakova T.A., Shatokhina A.V., Belokon Yu.S., Oreshkova N.V., Putintseva Yu.A., Sharov V.V., Kuzmin D.A., Krutovskiy K.V. Development of Siberian microsatellite markers of cedar pine (Pinus sibirica Du Tour) sequencing results of a genome-wide boiler de novo. Genetics, 2016, no. 12 (52), pp. 1418-1427. DOI: 10.7868/S0016675816120031. (In Russ.)
3. Blanc-Jolivet C., Yanbaev Yu., Kersten B., Degen B.A. Set of SNP markers for timber tracking of Larix spp. in Europe and Russia. Forestry, 2018, vol. 91, pp. 614– 628. DOI: 10.1093/forestry/cpy020.
4. Bratilova N.P., Kalinin A.V., Krot T.L. Comparative analysis of growth and adaptive abilities of cedar pines in the conditions of the south of Central Siberia. Fruit growing, seed production, introduction of woody plants: mat. of int. conf. Krasnoyarsk, 2003, no. 6, pp. 13-15. (In Russ.)
5. Doyle J.J., Doyle J.L. Isolation of plant DNA from fresh tissue. Focus, 1990, vol. 12, pp. 13-15. DOI: 10.2307/2419362.
6. Evanno G., Regnaut S., Goudet J. Detecting the number of clusters of individuals using the software STRUCTURE: A simulation study. Molecular Ecology, 2005, vol. 14, pp. 2611–2620. DOI: 10.1111/j.1365-294X.2005.02553.x.
7. FAO. The state of the world’s forest genetic resources. Commission on genetic resources for food and agriculture. Rome, 2014. 277 p.
8. Goudet J. Hierfstat, a package for R to compute and test hierarchical F-statistics. Molecular Ecology Notes, 2005, vol. 5, pp. 184-186. DOI: 10.1111/j.1471-8286.2004.00828.x.
9. Graudal L., Loo J., Fady B., Vendramin G., Aravanopoulos F.A., Baldinelli G., Bennadji Z., Ramamonjisoa L., Changtragoon S., Kjær E.D. Indicators of the genetic diversity of trees – State, Pressure, Benefit and Response. Rome: FAO, 2020. 92 p. DOI: 10.4060/cb2492en.
10. Gribkov A.V., Shchur A.V., Kuzmenkin D.V. Cedar forests of Altai under threat: problems of protection and use, recommendations for sustainable forest management. Moscow, 2014. 64 p. (In Russ.)
11. Hamrick J.L., Godt M.J., Sherman-Broyles S.L. Factors influencing levels of genetic diversity in woody plant species. New Forests, 1992, vol. 6, pp. 95-124. DOI: 10.1007/978-94-011-2815-5_7.
12. Hoban S., Bruford M., D’Urban J.J., Lopes-Fernandes M., Heuertz M., Hohenlohe P.A., Paz-Vinas I., Sjögren-Gulve P., Segelbacher G., Vernesi C., Aitken S., Bertola L.D., Bloomer P., Breed M., Rodríguez-Correa H., Funk W.C., Grueber C.E., Hunter M.E., Jaffe R., Liggins L., Mergeay J., Moharrek F., O’Brien D., Ogden R., Palma-Silva C., Pierson J., Ramakrishnan U., Simo-Droissart M., Tani n., Waits L., Laikre L. Genetic diversity targets and indicators in the CBD post-2020 Global Biodiversity Framework must be improved. Biological Conservation, 2020, vol. 248, art. no. 108654. DOI: 10.1016/j.biocon.2020.108654.
13. Hubisz M.J., Falush D., Stephens M., Pritchard J.K. Inferring weak population structure with the assistance of sample group information. Molecular Ecology, 2009, vol. 9, pp. 1322–1332. DOI: 10.1111/j.1755-0998.2009.02591.x.
14. Jombart T., Devillard S., Dufour A.B., Pontier D. Revealing cryptic spatial patterns in genetic variability by a new multivariate method. Heredity, 2008, vol. 101, pp. 92- 103. DOI: 10.1038/hdy.2008.34.
15. Krutovskiy K.V., Politov D.V., Altukhov Yu.P., Milyutin L.I., Kuznetsova G.V., Iroshnikov A.I., Vorobyev V.N., Vorobyeva N.A. Genetic variability of the Siberian stone pine. Message IV. Genetic diversity and the degree of genetic differentiation between populations. Genetics, 1989, vol. 25, no. 11, pp. 2009-2032. (In Russ.)
16. Krylov G.V., Talantsev N.K., Kozakova N.F. Cedar. Moscow: Forest Industry, 1983. 216 p. (In Russ.)
17. Kuznetsova G.V. The experience of creating a clone plantation of cedar pines in the Krasnoyarsk forest-steppe. Conifers of the boreal zone, 2007, vol. XXIV, no. 2-3, pp. 217-224. (In Russ.)
18. Li Y-L., Liu J-X. StructureSelector: A web-based software to select and visualize the optimal number of clusters using multiple methods. Molecular Ecology Resources, 2018, vol. 18, iss. 1, pp. 176-177. DOI: 10.1111/1755-0998.12719.
19. Matveeva R.N., Butorova O.F. Collection of clones, semis, and various morphological forms of Siberian cedar on the SibGU plantation (south of Central Siberia). Krasnoyarsk: SibGU, 2012. 47 p. (In Russ.)
20. Matveeva R.N., Milyutin L.I., Butorova O.F., Bratilova N.P. Selection of Siberian cedar trees of high reproductive capacity on a geographical forest seed plantation. IVUZ. Lesnoy zhurnal, 2017, no. 2, pp. 9-17. DOI: 10.17238/issn0536-1036.2017.2.9. (In Russ.)
21. Mitrofanov S.V. The influence of the method of grafting Siberian cedar on the growth and survival of the graft. Forestry, 2013, no. 2, pp. 120-122. (In Russ.)
22. Naoko T., Nei M., Tamura K. POPTREE2: Software for constructing population trees from allele frequency data and computing other population statistics with Windows interface. Molecular biology and evolution, 2010, vol. 27, no. 4, pp. 747-52. DOI: 10.1093/molbev/msp312.
23. Nei M. Genetic distance between populations. American Naturalist, 1972, vol. 106, pp. 283-291. DOI: 10.1086/282771.
24. Oreshkova N.V., Belokon M.M., Zhamyansuren S. Genetic diversity, population structure and differentiation of Siberian larches, Gmelin and Kayander according to SSR markers. Genetics, 2013, vol. 49, no. 2, pp. 204-213. DOI: 10.1134/S1022795412120095. (In Russ.)
25. Oreshkova N.V., Sedelnikova T.V., Pimenov A.V., Efremov S.P. Genetic structure and differentiation of marsh and drywall populations of Siberian cedar pine (Pinus sibirica Du Tour) by nuclear microsatellite loci. Genetics, 2014, vol. 50, no. 9, pp. 934- 941. DOI: 10.1134/S1022795414090105. (In Russ.)
26. Oreshkova N.V., Sedel’nikova T.S., Efremov S.P., Pimenov A.V. Genetic polymorphism of Siberian Stone Pine (Pinus sibirica Du Tour) in Kuznetsk Alatau. Contemporary Problems of Ecology, 2020, vol. 13, no. 6, pp. 569-576. DOI: 10.1134/S1995425520060116.
27. Padutov V.E., Khotyleva L.V., Baranov O.Yu., Ivanovskaya S.I. Genetic effects of forest ecosystem transformation. Ecological Genetics, 2008, vol. 6, no. 1, pp. 3-11. DOI: 10.17816/ecogen613-11. (In Russ.)
28. Peakall R., Smouse P.E. GenAlEx V6: Genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes, 2006, vol. 6, no. 1, pp. 288-295. DOI: 10.1111/j.1471-8286.2005.01155.x.
29. Petrova E.A., Belokon Yu.S. Comparative analysis of genetic polymorphism in populations of Siberian cedar (Pinus sibirica Du Tour) from the Ural and Altai-Sayan mountain regions. Problems of Botany of Southern Siberia and Mongolia, 2020, vol. 19, no. 2, pp. 81-86. DOI: 10.14258/pbssm.2020080. (In Russ.)
30. Pritchard J., Stephens M., Donnelly P. Inference of population structure using multilocus genotype data. Genetics, 2000, vol. 155, pp. 945-959. DOI: 10.1093/genetics/155.2.945.
31. Putenikhina K.V. Variability of generative organs of Siberian cedar during introduction in the Bashkir Pre-Urals: breeding possibilities. Materials of the 4th int. meet. dedic. to conservation of Siberia forest genetic resources. Krasnoyarsk, 2015, pp. 146-148. (In Russ.)
32. Rai K.C., Ginwal H.S. Microsatellite analysis to study genetic diversity in Khasi Pine (Pinus kesiya Royle ex. Gordon) using chloroplast SSR markers. Silvae Genetica, 2018, vol. 67, pp. 99-105. DOI: 10.2478/sg-2018-0014.
33. R Core Team. R: A Language and Environment for Statistical Computing; R Foundation for Statistical Computing. Vienna, 2013.
34. Semechkin I.V., Polikarpov N.P., Iroshnikov A.I. Cedar forests of Siberia. Novosibirsk: Nauka, 1985. 257 p. (In Russ.)
35. Semerikov V., Semerikova S., Putintseva J., Tarakanov V., Tikhonova I., Vidyakin A., Oreshkova N., Krutovsky K. Colonization history of Scots pine in Eastern Europe and North Asia based on mitochondrial DNA variation. Tree Genetics & Genomes, 2018, vol. 14, art. no. 8. DOI: 10.1007/s11295-017-1222-0.
36. Shah S., Qijing L., Jian Y., Shengwang M., Guang Z., Yuanyuan L., Khan D., Ahmad A., Saeed S., Mannan A. Potential geo-distribution of Pinus sibirica demonstrated by climatic similarity between Western Siberia and Northeast China. Journal of Animal and Plant Sciences, 2019, vol. 29, no. 4, pp. 1-8.
37. Sheller M., Tóth E.G., Mikhaylov P., Kulakov S., Kulakova N., Shilkina E.A., Ibe A., Sukhikh T., Blanc-Jolivet C. Genetic diversity and structure of Siberian Stone Pine (Pinus sibirica Du Tour) populations. Silvae Genetica, 2023а, vol. 72, pp. 25-33. DOI: 10.2478/sg-2023-0003.
38. Sheller M., Tóth E.G., Ciocîrlan E., Mikhaylov P., Kulakov S., Kulakova N., Melnichenko N., Ibe A., Sukhikh T., Curtu A.L. Genetic Diversity and Population Structure of Scots Pine (Pinus sylvestris L.) in Middle Siberia. Forests, 2023b, vol. 14, iss. 1, art. no. 119. DOI: 10.3390/f14010119.
39. Shilkina E.A., Ibe A.A., Sheller M.A., Sukhov T.V. Genetic differentiation of populations of Picea obovata L. in the regions of Siberia. Conifers of the boreal zone, 2019, vol. XXXVII, no. 1, pp. 68-73. (In Russ.)
40. Shuvaev D.N., Ibe A.A. Genetic structure and postglacial recolonization of Pinus sibirica Du Tour in the West Siberian Plain, inferred from nuclear microsatellite markers. Silvae Genetica, 2021, vol. 70, pp. 99-107. DOI: 10.2478/sg-2021-0008.
41. Șofletea N., Mihai G., Ciocîrlan E., Curtu A.L. Genetic Diversity and Spatial Genetic Structure in Isolated Scots Pine (Pinus sylvestris L.) Populations Native to Eastern and Southern Carpathians. Forests, 2020, vol. 11, art. no. 1047. DOI: 10.3390/f11101047.
42. Spielman D., Brook B.W., Frankham R. Most species are not driven to extinction before genetic factors impact them. Proceedings of the National Academy of Sciences, 2004, no.101, pp. 15261-15264. DOI: 10.1073/pnas.0403809101.
43. Titov E.V. Plantation cultivation of cedar pines. Voronezh: VGLTA, 2004. 165 p. (In Russ.)
44. Titov E.V. Siberian cedar is the king of the Siberian taiga. Moscow: Kolos, 2007. 152 p. (In Russ.)
Review
For citations:
Sheller M.A., Ibe A.A., Sukhikh T.V., Pastukhova A.M., Shcherba Yu.E. Genetic diversity of Siberian stone pine (Pinus sibirica Du Tour) in provenance trial plantation. Izvestia Sankt-Peterburgskoj lesotehniceskoj akademii. 2026;(258):51-68. (In Russ.) https://doi.org/10.21266/2079-4304.2026.258.51-68
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